| Congresso Brasileiro de Microbiologia 2023 | Resumo: 50-1 | ||||
Resumo:Antarctic soils are among the oldest, coldest, and driest found on the planet, their formation is restricted to areas free of snow and ice and corresponds to about 0.35% of their total area. Despite the adverse conditions, studies indicate a great diversity of microbial life in these soils, with varied biotechnological applications. That said, the objective of this work was to isolate fungi from pesticide-resistant Antarctic soils and rocks in order to check their potential to biodegrade agrochemicals. Soil samples were collected on the islands of the South Shetland archipelago during the Brazilian Expedition to the Antarctic Continent, OPERANTAR XXXVI (summer 2017/2018). The isolation protocol was performed with 12 samples from different areas, these called: Crater Lake, Widened, Rose Mary Lake, Yellow Point Casa do Cachorro, Yellow Point Below Morro da Cruz, Yellow Point Punta Plaza1, Fumarole Bay P1, Fumarole Bay P2, Costão Punta Plaza 1, Costão Punta Plaza 2, Costão Baleia, Costão Punta Ullmann . The samples were submitted to the serial dilution method in 0.9% NaCl saline solution and an aliquot of 1000 μL of the dilutions from 10-1 to 10-2, was used for plating in Malt Extract Agar and Imidacloprid (EMI) medium, supplemented with chloramphenicol at 200mg/L to prevent bacterial growth and supplemented with 200mg/L of the 20% pesticide Imidacloprid. The plates were incubated at 15 ºC for 60 days and aftergrowth, the microorganisms were purified in new culture media and preserved in 20% glycerol solution and stored in an ultrafreezer. The tolerance/resistance activity was observed from the growth in plates with EMI medium. A total of 28 filamentous fungi and 5 yeasts were isolated. For samples Crater Lake 21,4%, Widened 10,7%, Yellow Point Casa do Cachorro 17,9%, Yellow Point Below Morro da Cruz 10,7%, Yellow Point Punta Plaza 10,7%, Fumarole Bay P1 14,3%, Fumarole Bay P2 3,6%, Costão Punta Plaza01 7,1%, Costão Punta Plaza02 3,6%. And for yeasts, Costão Baleia 80%, Costão Punta Plaza01 20%. Of the isolates, it was possible to identify with microculture Penicillium sp. (5), Antarctomyces sp. (3), Thelebolus sp. (2), Pseudeurotium sp. (3), and Cladosporium sp. (2). In this way, it was possible to start the construction of a microbiological library which can be applied in future works with the perspective of biodegradation of agrochemicals and possible agricultural applications. Palavras-chave: EXTREMOPHILES, BIODEGRADATION, IMIDACLOPRID Agência de fomento:FAPEAL | |||||